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Hydrophilic polymethylmethacrylate hollow fibers for capillary electrophoresis of biomolecules

✍ Scribed by Shujun Chen; Milton L. Lee

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
93 KB
Volume
9
Category
Article
ISSN
1040-7685

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✦ Synopsis

Neutral hydrophilic polymethylmethacrylate PMMA hollow fibers were used as capillary electrophoresis columns. Efficient separations of proteins and nucleotides were obtained with the untreated columns. More than 230,000 theoretical plates were obtained for lysozyme variants. The uniformity and ultraviolet adsorption properties of the PMMA capillaries were evaluated. The percent Ε½ . relative standard deviation RSD of the PMMA hollow-fiber inner diameter was 5.2%. The RSD of the PMMA hollow-fiber outer diameter was 2.4%. PMMA hollow fibers are transparent above 250 nm. The electroosmotic flow characteristics of the hydrophilic PMMA were compared to those of fused silica and hydrophobic polypropylene. The fused silica and polypropylene columns exhibit relatively high electroosmotic flow compared to PMMA. In addition, comparisons of heat dissipation abilities between fused silica and PMMA were made. Overall, PMMA hollow fibers perform in capillary electrophoresis as well as fused silica capillaries at relatively low voltage.

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