Plexiglas column coatings for capillary electrophoresis of biomolecules

✍ Scribed by Xiaowen Shao; Yufeng Shen; Kim O'neill; Milton L. Lee

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 87 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:5<325::aid-mcs1>3.0.co;2-w

✦ Synopsis

In this article, Plexiglas primarily polymethylmethacrylate was used to Ž . modify fused silica capillary columns for capillary electrophoresis CE . The procedure was carried out using a dynamic coating method at room temperature. The whole process required only 20 min, and the resulting capillaries could be immediately used for the analysis of biomolecules. Greatly suppressed electroos-Ž . motic flow EOF was observed over a pH range of 3᎐10. Various biochemical standards including ribonucleotides, peptides, proteins, and DNA were successfully separated. More than 0.5 = 10 6 theoretical plates were obtained, and this efficiency changed little in more than 1 month of continuous use. Lower than ; 1.6% Ž . relative standard deviation in migration times 27 successive runs was observed for model proteins. The simplicity of the modification process allows this method to be suitable for modifying miniaturized electrophoresis channels or devices.