Polymethacryloxypropylhydrosiloxane deactivation as pretreatment of polymer-coated fused silica columns for capillary electrophoresis
✍ Scribed by A. Fridström; N. Lundell; L. Nyholm; K.E. Markides
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 128 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
A new polymer, polymethacryloxypropylhydrosiloxane PMAHS , has been developed and used as both a deactivating layer and an intermediate layer for stable coating of an uncharged polymer on fused silica capillaries in capillary electrophoresis. The deactivation procedure is based on a siliconhydride dehydrocondensation reaction which produces a thin and heavily crosslinked siloxane resin on the fused silica surface. The resin effectively covers any unreacted silanols, while the methacrylic substituents of the deactivation layer provide surface wettability and reaction sites for covalent binding of a polymeric top layer known to facilitate separations of charged biomolecules. In this study, polyacrylamide was statically coated and crosslinked to the deactivation polymer. The PMAHSdeactivated columns with crosslinked polyacrylamide coatings were found to give an electroosmotic flow of -0.4 = 10 y4 cm 2 V y1 s y1 , independent of pH, between pH 2.5 and 9.2 Four basic proteins were used to evaluate the performance of the columns. The migration times were reproducible with a relative standard deviation of -0.5%. In addition, the efficiency of the crosslinked polyacrylamide column was stable over at least 5 days of harsh testing.