✦ LIBER ✦

Polymerase chain reaction products analyzed by charge detection mass spectrometry

✍ Scribed by Jocelyn C. Schultz; Christopher A. Hack; W. Henry Benner

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 85 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990115)13:1<15::aid-rcm439>3.0.co;2-a

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✦ Synopsis

Charge detection mass spectrometry (CDMS) has been used to rapidly analyze polymerase chain reaction (PCR) products with a minimum of sample cleanup after the PCR reaction. PCR products of 1525 base pairs (bp), 1982 bp and 2677 bp were detected by CDMS, where simultaneous measurement of the charge and velocity of an electrostatically accelerated ion allows a mass determination of the ion. We extended the investigation to a longer double-stranded, linear DNA sample by also analyzing linearized pBR322 (4361 bp). Positive ion mass spectra and ion charge-state distributions were obtained for these electrosprayed DNA samples. Instrumental conditions were studied using a commercially available, linearized pUC18 sample (2682 bp).

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