Accurate monitoring and identification of Legionella species, the causative agents of Legionnaires' and other diseases, in environmental water sources is an important public health issue. Traditional culture methods often lack the sensitivity and specificity that can be attained using the polymerase
✦ LIBER ✦
Analysis of Polymerase Chain Reaction-Amplified DNA Products by Mass Spectrometry Using Matrix-Assisted Laser Desorption and Electrospray: Current Status
✍ Scribed by M.J. Doktycz; G.B. Hurst; S. Habibigoudarzi; S.A. Mcluckey; K. Tang; C.H. Chen; M. Uziel; K.B. Jacobson; R.P. Woychik; M.V. Buchanan
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 969 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0003-2697
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Asymmetric polymerase chain reaction (PCR) simplifies the application of biotin-streptavidin based purification formats for PCR products in matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis. The use of an excess of non-biotinylated primer improves the turnover of