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Polymer-shielded dye-affinity chromatography

✍ Scribed by Bo Mattiasson; Igor Yu. Galaev; Nandita Garg

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
611 KB
Volume
9
Category
Article
ISSN
0952-3499

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✦ Synopsis

Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(viny1pyrrolidone) or poly(viny1 alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.

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