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Fungal pectic enzyme fractionation by dye affinity chromatography

✍ Scribed by Silvia A. Camperi; Mariano Grasselli; Osvaldo Cascone

Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
299 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

A dye affmity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographic matrix, the retained pectin lyase could be quantitatively eluted with a salt gradient.

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