Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography

✍ Scribed by R. F. Boyer; T. L. Allen; P. A. Dykema

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 387 KB
• Volume: 29
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260290206

✦ Synopsis

Eight chemically modified cellulose supports were tested for their ability to adsorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. These supports apparently owe their resolving capacity to both ion exchange and biospecific binding effects; however, the relative importance of each effect is unknown. These observations form the basis for a new cellulase fractionation technique, combined ion exchange-affinity chromatography. CCC 0006-3592/87/020 1 76-04$04.00 * The batch adsorption experiments were performed as described in the Methods section. After centrifugation protein measurements were made on the supernatant by absorbance at 280 nm. The results are the average of five experiments.