Characterisation of Fractions Obtained by Isoamylolysis and Ion-exchange Chromatography of Cationic Waxy Maize Starch
✍ Scribed by Robin Manelius; Hannu Maaheimo; Kari Nurmi; Eric Bertoft
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 163 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0038-9056
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✦ Synopsis
Despite the work carried out so far, a more detailed knowledge of the location of the substituents within the granules and distribution between the starch components would help to further improve and optimise the modification processes and to develop new products and applications of starch. Therefore, there is a continuous need for the development of analytical methods for the characterisation of modified starches. In what follows an ion-exchange chromatography method is described, by which the charged dextrins released by isoamylolysis of cationic starch can be isolated and further studied. Cationic starches, commonly substituted with a hydroxypropyl trimethylammonium group and widely used in the paper industry, can be produced by either a wet-cationisation [22] or a dry-cationisation method [23,24]. The samples under study in this work were wet-(WC) and drycationised (DC) waxy maize starch with a degree of substitution (DS) of 0.046 and were partly characterised previously [20].
Materials and Methods
2.1 Starches and enzymes
Preparation and characterisation of the wet-and drycationised waxy maize starches used has been described previously [20]. Both samples had a degree of substitution (DS) of 0.046. Isoamylase from Pseudomonas amyloderamosa EC 3.2.1.68