Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(viny1pyrrolidone) or poly(viny1 alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer sh
Polymer displacement in dye-affinity chromatography
✍ Scribed by Igor Yu. Galaev; Pär Arvidsson; Bo Mattiasson
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 565 KB
- Volume
- 710
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Displacement chromatography was demonstrated to perform separations efficiently under mass-overloaded conditions, offering advantages such as increased product recovery and purity, superior resolving power, and concentration and purification in a single processing step. The use of water-soluble poly
In this paper a new graphical method, derived from the Steric Mass Action model of non-linear ion-exchange chromatography, is presented for the determination of solute affinity in ion exchange displacement systems. The affinity of solutes in these systems is described by a dynamic affinity parameter
Cibacron Brilliant Blue BR-P, a structural isomer of Cibacron Blue 3G-A (the chromophore in Blue Dextran), and Procion Brilliant Blue MR, Cibacron-Brilliant. Blue BR-P without the sulfonated bet& moiety, were covalently attached to Sepharose CL-6B by the triazine rings. The use of these materials fo
Continuous beds derivatized with three triazine dyes (Cibacron Blue 3G-A, Pricon Red HE3B and Pricon Red H-3B) were used for chromatographic purification of dehydrogenases from yeast enzyme concentrate. All three columns, which were prepared by a very simple and cost-effective method, provided stron
Hollow-fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G-A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity