Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: Mesh sizes, separation and dispersion
β Scribed by Chunhung Wu; Mark A. Quesada; Dieter K. Schneider; Raymond Farinato; F. William Studier; Dr. Benjamin Chu
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 726 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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An optimized procedure for the fast separation of DNA sequencing fragments in short bare fused-silica capillaries filled with highly alkaline solutions of replaceable linear polyacrylamide is presented. High denaturing abilities of the separation media at pH values over 12 are the main reason for th
## Abstract By employing linear polyacrylamide (LPA) as a sieving matrix, oligonucleotides and DNA sequencing fragments have been separated by capillary electrophoresis. A commercially available apparatus equipped with a laserβinduced fluorescence (LIF) detection system has been utilized, but the c