A new concept for sequencing DNA by capillary electrophoresis
β Scribed by Dr. Jaan Noolandi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 254 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
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In capillary electrophoresis, covering the inner capillary surface with a coating is an efficient way to minimize both the electroosmotic flow and sorption of w analytes on the capillary wall. We modified the procedure by Cobb et al. Anal. Ε½ .x Chem. 62, 2478 1990 for preparing wall coating to perm
## Abstract We assessed the feasibility of highβspeed DNA sequencing by tubeβbased capillary electrophoresis (TCE) with electrokinetic sample injections. We developed a waterβcirculated TCE system to control the capillary temperature precisely. Using this system and a readyβmade sieving matrix at 5