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Polarographic behavior of cocarboxylase in Britton—Robinson buffer

✍ Scribed by T. Vergara; D. Marín; J. Vera; A. Sarabia

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
353 KB
Volume
25
Category
Article
ISSN
0013-4686

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✦ Synopsis

The polarographic behavior of cocarboxylase in the dme in a Britton-Robinson buffered media, with a constant ionic strength (p =OS M), along a pH range 2-12, is studied. Two diffusion cathodic waves, a catalytic wave and an anodic one are found. The ply and pK' (polarographic pK) of this substance are calculated both polarographically and spextrophotometrically. The electron number involved in the reduction of cocarboxylase is calculated by coulometry with a pool mercury electrode. On the basis of the results obtained and its discussion a reduction mechanism in dme as a function of the pH is proposed.

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