Behavior of erythrocytes in phosphate buffer systems

✍ Scribed by Janis R. Phillips; Donald E. Cadwallader

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 380 KB
• Volume: 60
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600600706

✦ Synopsis

Hemolytic behavior of human erythrocytes in sodium and potassium phosphate buffer systems was invest;gated. The data were used to calculate hemoly tic i values for the buffer components at various pH values. The experimental i values were lower than those predicted by a theoretical equation, and the deviations were attributed to changes in pH causing alterations in the permeability of the red cell membrane to the anions and/or cations in solution or changes in the red cell contents. Alkaline solutions appear to be more favorable environments for the red blood cell under the conditions studied. The increased osmotic fragility (low hemoly tic i values) at lower pH values was attributed to an increase in the osmotic activity of the cell contents and subsequent movement of water into the cell.