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Point mutation in the steroid-binding domain of the androgen receptor gene in a family with complete androgen insensitivity syndrome (CAIS)

✍ Scribed by Sibylle Jakubiczka; Edmond A. Werder; Peter Wieacker

Publisher: Springer
Year: 1992
Tongue: English
Weight: 325 KB
Volume: 90
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00220088

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✦ Synopsis

An exonic single nucleotide substitution in the human androgen receptor gene (hAR) could be detected in an Italian family with two children affected by complete androgen insensitivity syndrome (CAIS), also called testicular feminization. This mutation leads to a guanine to adenine transition in exon 5, changing the sense of the codon from methionine (ATG) to valine (GTG). As this mutation abolishes a NcoI restriction site, a rapid test for the mutation can be performed by digestion of the polymerase chain reaction products with this enzyme. Previous results of indirect gene diagnosis in this family could be confirmed by this method.

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