## Communicated by Stylianos E. Antonarakis fied by polymerase chain reaction (PCR) ace cording to Saiki et al. (1988) using the intronic primers of Lubahn et al. (1989). Routinely, 32 cycles were run in an Intelligent Heating Block (Biometra) after a first denaturation step for 5 min at 93ยฐC, 60
Amber mutation creates a diagnosticMaeI site in the androgen receptor gene of a family with complete androgen insensitivity
โ Scribed by Trifiro, Mark ;Prior, R. Lynn ;Sabbaghian, Nelly ;Pinsky, Leonard ;Kaufman, Morris ;Nylen, Edward G. ;Belsham, Denise D. ;Greenberg, Cheryl R. ;Wrogemann, Klaus
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 686 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0148-7299
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Mutations of the androgen receptor gene causing androgen insensitivity syndrome in 46, XY individuals, result in phenotypes ranging from complete female to ambiguous genitalia to males with minor degrees of undervirilization. We studied two Brazilian brothers with partial androgen insensitivity synd
p53 protein expression of 30 hormone-refractory locally recurrent prostate cancers was compared with their matched untreated primary tumour specimens. In addition, androgen receptor (AR) gene amplification and p53 protein immunostaining were compared. p53 positivity increased during hormonal therapy