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Plasmid replication functions

✍ Scribed by Lurz, R. ;Danbara, H. ;Rückert, B. ;Timmis, K. N.


Publisher
Springer
Year
1981
Tongue
English
Weight
819 KB
Volume
183
Category
Article
ISSN
0026-8925

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Plasmid replication functions
✍ Synenki, Richard M. ;Nordheim, Alfred ;Timmis, Kenneth N. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 920 KB

Replicating DNA molecules of the mini R6-5 plasmid, pKTO71, were purified by equilibrium centrifugation in two successive ethidium bromide-caesium chloride gradients, converted to linear forms by cleavage with either HindIII or BglII restriction endonuclease, and examined in the electron microscope.

Plasmid pSC101 replication in integrativ
✍ Felton, Jeffrey ;Wright, Andrew 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 251 KB

Maintenance of plasmid pKC17, a derivative of plasmid pSC101, in E. coli requires a functional dnaA gene product. This was demonstrated by segregation experiments using an E. coli dnaA46 mutant, at various temperatures. Growth of this mutant at elevated temperature was allowed by the presence of a P