We isolated and characterized mutants of ColE2 with increased copy number (cop) and those with reduced sensitivity to the wild-type incA gene (inc). Both types of mutations were single-base substitutions in the incA region and simultaneously increased the plasmid copy number and reduced the inhibito
Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication
✍ Scribed by Light, Janice ;Molin, Søren
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 653 KB
- Volume
- 184
- Category
- Article
- ISSN
- 0026-8925
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## Abstract The replication of R124, and a copy mutant derivative of it, was measured with respect to dependence on the host DnaA, DnaB, DnaC, DnaE, DnaG, and Po1A gene products. Both plasmids replicated under conditions where the DnaA gene product was inactivated or where the polymerising activity
Five different copA copy mutants of plasmid R1 have been identified by nucleotide sequencing. Independent measurements of the activities of the mutant inhibitor RNA and of the mutant target properties were carried out using several different methods. Correlation of these measurements with the locati