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Host components required for the replication of the resistance plasmid R124 and a copy mutant derivative

✍ Scribed by J. J. Pritchard; Dr. R. J. Rowbury

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
825 KB
Volume
20
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The replication of R124, and a copy mutant derivative of it, was measured with respect to dependence on the host DnaA, DnaB, DnaC, DnaE, DnaG, and Po1A gene products. Both plasmids replicated under conditions where the DnaA gene product was inactivated or where the polymerising activity of the PolA gene product was reduced. In contrast, neither plasmid replicated to any appreciable extent, if the DnaB, DnaC, DnaE or DnaG gene products were inactivated. R124 integratively suppressed the lesion of the dnaA mutant but the copy mutant derivative had only a very weak suppressing effect. Neither plasmid suppressed the lesions of any of the other dna mutants.

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Isolation and characterization of the mi
Isolation and characterization of the minimal fragment required for autonomous replication (β€œBasic replicon”) of a copy mutant (pKN102) of the antibiotic resistance factor R1
✍ Kollek, Regine ;Oertel, Wolfgang ;Goebel, Werner πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 669 KB

The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the "bas