Plasmid deletion formation in Bacillus subtilis
β Scribed by Ad A.C.M. Peijnenburg; Sierd Bron; Gerard Venema
- Book ID
- 119078949
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 867 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0147-619X
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π SIMILAR VOLUMES
A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived
Various alterations (deletions, additions, inversions) were introduced into portions of pC194/B. subtilis or pC194/phi 105 hybrid plasmid molecules which are homologous to the DNA of recipients in transformation. These plasmids are stably maintained in transformations of recombination deficient cell
Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr
The plasmid pKBT1 was derived by in vivo recE4-independent recombinational event(s) yielding a structure containing regions of plasmid and chromosomal origin. BamHI digests of plasmid pUB110 (Kanr/Neor) and Bg/II digests of pTL12 (Tmpr, leuA) were mixed, ligated and used to transform competent cells