𝔖 Bobbio Scriptorium
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Plasmid curing in Bacillus subtilis

✍ Scribed by Nancy Fowler Edger; Karen Orzech McDonald; William F. Burke Jr.


Book ID
109311734
Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
193 KB
Volume
12
Category
Article
ISSN
0378-1097

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πŸ“œ SIMILAR VOLUMES


Plasmid transformation in Bacillus subti
✍ Bensi, G. ;Iglesias, A. ;Canosi, U. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 859 KB

A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived

Plasmid transformation in Bacillus subti
✍ Iglesias, A. ;Bensi, G. ;Canosi, U. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 573 KB

Various alterations (deletions, additions, inversions) were introduced into portions of pC194/B. subtilis or pC194/phi 105 hybrid plasmid molecules which are homologous to the DNA of recipients in transformation. These plasmids are stably maintained in transformations of recombination deficient cell

Plasmid transformation in Bacillus subti
✍ Iglesias, Antonio ;Ceglowski, Piotr ;Trautner, Thomas A. πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 666 KB

Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr