Plasmid curing in Bacillus subtilis
β Scribed by Nancy Fowler Edger; Karen Orzech McDonald; William F. Burke Jr.
- Book ID
- 109311734
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 193 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived
Various alterations (deletions, additions, inversions) were introduced into portions of pC194/B. subtilis or pC194/phi 105 hybrid plasmid molecules which are homologous to the DNA of recipients in transformation. These plasmids are stably maintained in transformations of recombination deficient cell
Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr