Plasma desorption mass spectrometry of proteins transferred from gels after sodium dodecyl sulphate–polyacrylamide gel electrophoresis
✍ Scribed by Klaus Klarskov; Peter Roepstorff
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 660 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1076-5174
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A new strategy for the characterization of Coomassie Brilliant Blue stained SDS-PAGE separated proteins by UV-MALDI-MS is reported. The proteins are extracted directly from the polyacrylamide gel by treatment with an organic solvent mixture consisting of formic acid, acetonitrile, isopropanol and wa
To the Editor-in-Chief Sir, Electroelution and passive elution of g-globulins from sodium dodecyl sulphate polyacrylamide gel electrophoresis gels for matrix-assisted laser desorption/ionisation time-offlight mass spectrometry Figure 1. (a) SDS-PAGE stained with Coomassie Brilliant Blue of intact hu
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