A new strategy for the characterization of Coomassie Brilliant Blue stained SDS-PAGE separated proteins by UV-MALDI-MS is reported. The proteins are extracted directly from the polyacrylamide gel by treatment with an organic solvent mixture consisting of formic acid, acetonitrile, isopropanol and wa
Electroelution and passive elution of γ-globulins from sodium dodecyl sulphate polyacrylamide gel electrophoresis gels for matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
✍ Scribed by Marina Galvani; Mahmoud Hamdan
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 130 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
To the Editor-in-Chief Sir, Electroelution and passive elution of g-globulins from sodium dodecyl sulphate polyacrylamide gel electrophoresis gels for matrix-assisted laser desorption/ionisation time-offlight mass spectrometry Figure 1. (a) SDS-PAGE stained with Coomassie Brilliant Blue of intact human g-globulins, (b) MALDI-TOF mass spectrum associated with the band on the right-hand side of the map.
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