Plant regeneration via somatic embryogenesis from protoplasts of six plant species related toCitrus
β Scribed by Hasan Basri Jumin, Nobumasa Nito
- Book ID
- 120780659
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 846 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0721-7714
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Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free
Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditio