𝔖 Bobbio Scriptorium
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Plant regeneration via somatic embryogenesis from protoplasts of six plant species related toCitrus

✍ Scribed by Hasan Basri Jumin, Nobumasa Nito


Book ID
120780659
Publisher
Springer
Year
1996
Tongue
English
Weight
846 KB
Volume
15
Category
Article
ISSN
0721-7714

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Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free

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Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditio