𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Plant regeneration from pea protoplasts via somatic embyogenesis

✍ Scribed by Renate Lehminger-Mertens; Hans-Jörg Jacobsen

Publisher
Springer
Year
1989
Tongue
English
Weight
698 KB
Volume
8
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

✦ Synopsis

Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA3 - containing medium.

📜 SIMILAR VOLUMES

Direct somatic embryogenesis and plant r
Direct somatic embryogenesis and plant regeneration from protoplasts ofBupleurum scorzonerifoliumWilld
✍ Xia Guang-min; Li Zhongyi; Guo Guang-qin; Chen Hui-min 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 581 KB

Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditio