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Direct somatic embryogenesis and plant regeneration from protoplasts ofBupleurum scorzonerifoliumWilld

✍ Scribed by Xia Guang-min; Li Zhongyi; Guo Guang-qin; Chen Hui-min

Publisher
Springer
Year
1992
Tongue
English
Weight
581 KB
Volume
11
Category
Article
ISSN
0721-7714

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✦ Synopsis

Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to direct embryo formation were investigated, and the nature of the callus used for protoplast preparation was found to be a critical factor. The osmotic concentration and the composition of the culture medium including the phytohormone combinations were also important.

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