𝔖 Bobbio Scriptorium
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Somatic embryogenesis and plant regeneration from cell suspensions ofExacum affine

✍ Scribed by Holger Ørnstrup; Jens Peter Mølgaard; Birger Farestveit

Publisher
Springer
Year
1993
Tongue
English
Weight
850 KB
Volume
35
Category
Article
ISSN
0167-6857

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✦ Synopsis

Somatic embryos were produced in seven cultivars of Exacum affine Balf. using flower buds and peduncles as explants. Flowering plants were produced from five of the cultivars, and no visible mutations were detected. The best medium for callus induction and growth was MS supplemented with 9.0 ~M 2,4-dichlorophenoxyacetic acid and either zero or 0.089 I~M BA. Callus suspensions were made by passing the callus through a 100 I~m sieve. The best embryo regeneration was achieved on growth regulator-free medium. Callus and embryos could be grown in liquid medium.

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