High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis inCatharanthus roseus

✍ Scribed by Suk W. Kim; Nam H. Song; Kyung H. Jung; Sang S. Kwak; Jang R. Liu

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 751 KB
• Volume: 13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232629

✦ Synopsis

A system for high frequency plant regeneration from cell suspension cultures in Catharanthus roseus is described. Calli were obtained from anthers cultured on Murashige and Skoog's medium supplemented with 1 mgl(-1) α-naphthaleneacetic acid and 0.1 mgl(-1) kinetin. After the second subculture on solid medium, embryogenic callus was identified and transferred to liquid medium to initiate suspension cultures. Cells dispersed finely in the medium were subcultured at 14-day intervals. Upon plating onto the basal medium, yellowish compact colonies proliferated from the cells and more than 80% of them gave rise to somatic embryos. Subsequently, plantlets developed from the embryos. Both the plantlets and the source plants showed the normal somatic chromosome number of 2n=2x=16.