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Plant regeneration ofSolanum lycopersicoidesDun. from stem explants, callus and suspension cultures

โœ Scribed by L. W. Handley; K. C. Sink


Publisher
Springer
Year
1985
Tongue
English
Weight
673 KB
Volume
5
Category
Article
ISSN
0167-6857

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โœฆ Synopsis


Protocols were established for achieving plant regeneration from stem internode, callus, and cell suspension cultures of Solanum lycopersicoides Dun. Two accessions of S. lycopersicoides exhibited different responses as to callus formation on various media, requirement of gibberellic acid for shoot regeneration, and ability to grow in suspension culture. The optimum medium for initiation and maintenance of cell suspension cultures was Murashige and Skoog [9] medium with 15 mgl -I NAA. For shoot regeneration, of three cytokinins tested, zeatin was found most effective relative to number, rapidity of response and overall quality of shoots. Shoot regeneration from stem explants, callus and suspension cultures was optimum on MS + 3.0 mg 1-1 zeatin + 0.1 mg1-1 gibberellic acid.


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