Plant regeneration ofSolanum lycopersicoidesDun. from stem explants, callus and suspension cultures
โ Scribed by L. W. Handley; K. C. Sink
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 673 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0167-6857
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โฆ Synopsis
Protocols were established for achieving plant regeneration from stem internode, callus, and cell suspension cultures of Solanum lycopersicoides Dun. Two accessions of S. lycopersicoides exhibited different responses as to callus formation on various media, requirement of gibberellic acid for shoot regeneration, and ability to grow in suspension culture. The optimum medium for initiation and maintenance of cell suspension cultures was Murashige and Skoog [9] medium with 15 mgl -I NAA. For shoot regeneration, of three cytokinins tested, zeatin was found most effective relative to number, rapidity of response and overall quality of shoots. Shoot regeneration from stem explants, callus and suspension cultures was optimum on MS + 3.0 mg 1-1 zeatin + 0.1 mg1-1 gibberellic acid.
๐ SIMILAR VOLUMES
Callus was obtained from mericarps of Bunium persicum Boiss. on MS medium supplemented with 2.0 mg/1 2,4-D and 4.0 mg/1 Kn. Small white clumps of compactly packed cells developed on the callus on a medium containing 1.0 mg/1 2,4-D and 0 mg/1 Kn. These cell clumps differentiated numerous globular emb
The possibility of plant regeneration from leaf tissue, callus and callus protoplasts of Lycium barbarum L. has been studied. Leaf segments were cultured on B5 medium (Gamborg et al. 1968) containing 1.5 mg/1 6-benzylaminopurine and 0.5 mg/1 ฮฑ-naphthaleneacetic acid. Regeneration of shoots was initi