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Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets

✍ Scribed by S. R. Bhat; B. V. Ford-Lloyd; J. A. Callow


Publisher
Springer
Year
1985
Tongue
English
Weight
262 KB
Volume
4
Category
Article
ISSN
0721-7714

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Isolation of protoplasts and vacuoles fr
✍ Elisabeth HΓ€usler; Maike Petersen; August Wilhelm Alfermann πŸ“‚ Article πŸ“… 1993 πŸ› Springer 🌐 English βš– 326 KB

In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r