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Callus formation from protoplasts of a sugarbeet cell suspension culture

✍ Scribed by László Szabados; Carina Gaggero

Publisher
Springer
Year
1985
Tongue
English
Weight
905 KB
Volume
4
Category
Article
ISSN
0721-7714

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✍ Elisabeth Häusler; Maike Petersen; August Wilhelm Alfermann 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 326 KB

In order to study the accumulation and transport of rosmarinic acid in suspension cells of Coleus blumei we established an efficient method to isolate protoplasts and vacuoles. Protoplasts were disrupted by an osmotic shock in a medium with basic pH containing ethylenediamine tetraacetic acid. The r