Plant regeneration from tissue cultures of Persian buttercup (Ranunculus asiaticusL.)

✍ Scribed by C. Pugliesi; M. Rabaglio; F. Cecconi; S. Baroncelli

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 344 KB
• Volume: 28
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00055506

✦ Synopsis

Different plant explants of Persian buttercup (Ranunculus asiaticus L.) were screened for callus induction and adventitious shoot regeneration on different media to establish totipotent cultures. Murashige & Skoog (MS) medium was used, supplemented with different concentrations of the following growth regulators: kinetin, benzyladenine (BA), naphthaleneacetic acid (NAA) and indoleacetic acid (IAA). Callus was induced and adventitious buds regenerated only from cotyledonary explants after 4-5 weeks. Subculture of the regenerated buds on the same basal medium in presence of gibberellic acid (GA3) and BA produced well-organized shoots. Rooting was obtained by transferring shoots to growth regulator-free MS medium. A high rate of shoot multiplication has been achieved on medium with high concentration of kinetin and long-day photoperiod. Finally the plants were successfully transferred to soil and grown in a greenhouse.