Plant Regeneration from Seedling Cotyledon Protoplasts
โ Scribed by Lu, D.Y.; Pental, D.; Cocking, E.C.
- Book ID
- 122232136
- Publisher
- Elsevier
- Year
- 1982
- Weight
- 561 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0044-328X
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Plant regeneration from protoplasts is a prerequisite to the production of modified plants using somatic hybridization and transformation. Whole plant regeneration was achieved from protoplasts isolated from seedling cotyledons of Stylosanthes guianensis, S. macrocephala and S. scabra, three economi
Cotyledon protoplasts were isolated from seedlings of Xinjiang muskelon (Cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified Miller medium. High frequency division of the protoplast-derived cells was observed. Agarose bead culture with B6S3 tobacco crown gall nurse