Plant regeneration from cotyledon protoplasts of glycine canescens and G. clandestina
โ Scribed by Hammatt, N.; Kim, H-I.; Davey, M.R.; Nelson, R.S.; Cocking, E.C.
- Book ID
- 124105403
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 940 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0168-9452
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Cotyledon protoplasts were isolated from seedlings of Xinjiang muskelon (Cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified Miller medium. High frequency division of the protoplast-derived cells was observed. Agarose bead culture with B6S3 tobacco crown gall nurse
Protoplasts were isolated from immature cotyledons of six cultivars of Glycine max L. and cultured in the KP8 liquid medium su-~lemented with 0.2 mg/L 2,4-D, I mg/L NAA and 0.5 mg/L ZT. The protoplasts started to divide after 3-5 days of culture. Sustained divisions resulted in mass production of ce