Plant regeneration from protoplasts of soybean (Glycine maxL.)

✍ Scribed by Zhi-ming Wei; Zhi-hong Xu

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 598 KB
• Volume: 7
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00269935

✦ Synopsis

Protoplasts were isolated from immature cotyledons of six cultivars of Glycine max L. and cultured in the KP8 liquid medium su-~lemented with 0.2 mg/L 2,4-D, I mg/L NAA and 0.5 mg/L ZT. The protoplasts started to divide after 3-5 days of culture. Sustained divisions resulted in mass production of cell colonies and small calli in 6 weeks. The calli further grew to 2-3 mm on the gelritesolidified K8 medium and were transferred onto the MSB medium with I mg/L 2,4-D and 0.25 mg/L BA, to obtain compact and nodular calli. Shoot formation was initiated on MSB medium with 0.15 mg/L NAA, and BA, KT and ZT, 0.5 mg/L of each, with or without 500 mg/L CH. It was followed by plant regeneration. So far, 87 plants have been regenerated from 4 c~ltivars, and normal seeds were obtained from them after transplanting into pots.