Picrate paper kits for determination of total cyanogens in cassava roots and all forms of cyanogens in cassava products

✍ Scribed by Bradbury, Meredith G; Egan, Sylvia V; Bradbury, J Howard

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 168 KB
• Volume: 79
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(19990315)79:4<593::aid-jsfa222>3.0.co;2-2

✦ Synopsis

The simple semiquantitative picrate method for the determination of total cyanogens in cassava ýour has been modiüed by increasing the concentration of the picrate solution used to make up the picrate papers, such that a linear Beer's Law relation between absorbance and cyanogen content is obtained over the range 0-800 mg HCN equivalents kg-1 cassava. The method has been adapted to determine the total cyanogen content of cassava roots and the results compared using the picrate method and the acid hydrolysis method for six diþ erent roots from üve cultivars. The agreement between the results is satisfactory. The simple method for determination of total cyanogens in cassava roots in the üeld is available in kit form. The methodology has been modiüed to allow determination of the three diþ erent forms of cyanogens present in cassava ýour, viz HCN/CN-, acetone cyanohydrin and linamarin. HCN/CN-is determined by the picrate method in which cassava ýour is reacted with 0.1 M sulphuric acid for 3 h at room temperature. HCN/CN-plus acetone cyanohydrin is also determined by the picrate method after treating cassava ýour with 4.2 M guanidine hydrochloride at pH 8 for 3 h at room temperature. A comparison has been made of the amounts of the three cyanogens present in six cassava ýour samples using the semiquantitative picrate and the acid hydrolysis methods. The agreement between the two methods is satisfactory, which shows that the new methodology works well. The picrate method for determination of the three cyanogens in cassava ýour is also available as a kit.