Physiological enhancement of immunoglobulin production of hybridomas in serum-free media
โ Scribed by Hiroki Murakami; Koji Yamada; Sanetaka Shirahata; Atshushi Enomoto; Shuichi Kaminogawa
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 938 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0920-9069
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The advantages of serum-free culture for the manufacture of recombinant biopharmaceuticals from mammalian cells are reviewed. The process favoured is fed-batch serum-free cell culture. This process is applicable to the majority of cell lines, is practical on the large scale, gives the lowest manufac
The effects of growth factors, such as insulin, transferrin, lactoferrin, ethanolamine, and selenium, on proliferation and IgM production of human-human hybridomas HB4C5 cells in a serum-free enriched RDF (eRDF) medium were studied. Among them, lactoferrin markedly stimulated proliferation and IgM p