Physico-chemical Characterization of Floridean Starch of Red Algae

✍ Scribed by Shukun Yu; Andreas Blennow; Maja Bojko; Finn Madsen; Carl Erik Olsen; Søren B. Engelsen

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 157 KB
• Volume: 54
• Category: Article
• ISSN: 0038-9056
• DOI: 10.1002/1521-379x(200202)54:2<66::aid-star66>3.0.co;2-b

✦ Synopsis

Physico-chemical Characterization of Floridean Starch of Red Algae

The current work reports on isolation and physico-chemical characterization of floridean starch from three species of agarophytic macro red algae. As determined by 1 H-NMR spectroscopy, the average chain length and degree of branching frequency of this starch were 18 and 4.8, respectively. According to its amylopectin chain length distribution obtained by Dionex analysis, the crystalline polymorph of floridean starch from the red alga Gracilariopsis lemaneiformis was deduced to be C-type and this was further supported from its X-ray crystallographic pattern. Enzymatic analysis of its glucose 6-phosphate content showed that floridean starch had a low level of covalently linked phosphate (1 nmol per milligram starch) and this was further confirmed by 31 P-NMR. The absorbance peak of floridean starch with iodine occurred at 527-530 nm and the blue value was low (0.1), indicating the absence of amylose, which was confirmed by differential scanning calorimetry (DSC). Floridean starch exhibited low gelatinization temperature, low viscosity, high clarity and little or no retrogradation upon repetitive freeze-thaw cycles, as studied by DSC and rapid viscosity analysis (RVA). These results are discussed in light of the functional properties and the structure of floridean starch.