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Physical linkage between 5 s, 16 s and 23 s ribosomal RNA genes in Bacillus subtilis

✍ Scribed by Colli, Walter; Smith, Issar; Oishi, Michio


Book ID
121966315
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
819 KB
Volume
56
Category
Article
ISSN
0022-2836

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Linkage of 5S RNA and 16S+23S RNA genes
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Episomes carrying limited regions of the chromosome where 5S RNA genes have previously been located are described. The DNA purified from each of these episomes contains one gene per molecule for each of the three ribosomal RNA species as shown by hybridization experiments.

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A 9.2 kb Pstl restriction fragment, repeated tandemly head-to-tail in the genome, contains the 5.8S, 16-18S, and 23-28S ribosomal RNA (rRNA) genes of Cephalosporium acremonium, a filamentous fungus used at the industrial scale for production of cephalosporin antibiotics. These rRNA genes were locate