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Phylogenetic diversification of immunoglobulin VH genes

โœ Scribed by G.W. Litman; L. Berger; K. Murphy; R. Litman; F. Podlaski; K. Hinds; C.L. Jahn; G. Dingerkus; B.W. Erickson

Book ID
119076295
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
1015 KB
Volume
8
Category
Article
ISSN
0145-305X

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## Polymerase chain reaction 2 Materials and methods ### 2.1 Cell isolation and DNA extraction Granulocytes were isolated from peripheral blood of two unrelated individuals, A and B, on a Ficoll-Paque (G-S Chemical MFG. Corp.) gradient. High-molecular weight genomic DNA was extracted from the gr