VH replacement in rearranged immunoglobulin genes
β Scribed by John M. Darlow; David I. Stott
- Book ID
- 108781595
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 307 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0019-2805
No coin nor oath required. For personal study only.
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## Polymerase chain reaction 2 Materials and methods ### 2.1 Cell isolation and DNA extraction Granulocytes were isolated from peripheral blood of two unrelated individuals, A and B, on a Ficoll-Paque (G-S Chemical MFG. Corp.) gradient. High-molecular weight genomic DNA was extracted from the gr