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Photoregulation of the Formation and Dissociation of a DNA Duplex by Using the cis–trans Isomerization of Azobenzene

✍ Scribed by Hiroyuki Asanuma; Takanori Ito; Takayuki Yoshida; Xingguo Liang; Makoto Komiyama

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
89 KB
Volume
38
Category
Article
ISSN
0044-8249

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The duplex-forming activities of oligonucleotides can be photomodulated by incorporation of an azobenzene unit. Upon isomerizing the trans-azobenzene to the cis form by irradiation with UV light, the T m value of the duplex (with the complementary DNA) is lowered so that the duplex is dissociated. T

Rate constants of the thermal cis-trans
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Rate constants k iso of the thermal cis-trans isomerization of four 4,4'-nitro-aminoazobenzenes with different amino groups have been determined in homogeneous aprotic solvents and polyglykol oligomers, primarily by means of conventional flash photolysis. The rate constants have been correlated with