Photoregulation of the DNA Polymerase Reaction by Oligonucleotides Bearing an Azobenzene
β Scribed by Akira Yamazawa; Xingguo Liang; Hiroyuki Asanuma; Makoto Komiyama
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 79 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0044-8249
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π SIMILAR VOLUMES
The duplex-forming activities of oligonucleotides can be photomodulated by incorporation of an azobenzene unit. Upon isomerizing the trans-azobenzene to the cis form by irradiation with UV light, the T m value of the duplex (with the complementary DNA) is lowered so that the duplex is dissociated. T
## Abstract __Two deoxyuridine derivatives each bearing a monophosphate group at the 5βposition with a C3 linker, were incorporated into an oligonucleotide. By using this modified oligonucleotide, a bulge was formed at a predetermined position in a DNA substrate, and two monophosphate groups were p
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