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Phosphorylation of soluble pig epidermal proteins by endogenous calcium-activated, phospholipid-dependent protein kinase

✍ Scribed by H. Koizumi; T. Aoyagi; A. Ohkawara

Publisher: Springer-Verlag
Year: 1986
Tongue: English
Weight: 822 KB
Volume: 279
Category: Article
ISSN: 0340-3696
DOI: 10.1007/bf00417529

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✦ Synopsis

Endogenous calcium-activated, phospholipid-dependent protein kinase phosphorylates pig epidermal protein. Pig epidermis was homogenized and centrifuged at 30,000 X g for 30 min. The supernatant was incubated with or without calcium and phospholipid. A 97 kD soluble protein from pig epidermis was phosphorylated in the presence of calcium and phosphatidylserine. The phosphorylation reaction occurred immediately. With the use of two-dimensional polyacrylamide gel electrophoresis, it was shown that the 97 kD fragment was a basic protein, and that several small proteins were also phosphorylated. The characterization of these proteins is yet to be undertaken.

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