✦ LIBER ✦

Differential regulation of basic protein phosphorylation by calcium phospholipid and cyclic-AMP-dependent protein kinases

✍ Scribed by Glyn Dawson; Patrick McAtee

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 584 KB
Volume: 40
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240400302

No coin nor oath required. For personal study only.

✦ Synopsis

Myelin basic protein, an 80-kilodalton (kDa) protein in rat oligodendrocytes, and an 80-kDa basic protein in neuroblastoma x neonatal Chinese hamster brain explant hybrids were phosphorylated extensively when the cells were treated with either phorbol esters (TPA) or diacylglycerols (e.g., oleyoyl-acetylglycerol). TPA-stimulated phosphorylation was inhibited by pre-incubation with 50 pM psychosine (galactosyl-sphingosine), confirming that it is mediated through the phospholipid-dependent protein kinase C (PK-C). Surprisingly, phosphorylation of these proteins was inhibited by incubation of cells with agents which result in activation of cyclic-AMP-dependent protein kinase (dibutyryl cyclic AMP or forskolin). In contrast, phosphorylation of other nonbasic proteins, for example, the oligodendrocyte-specific 2',3'-cyclic nucleotide phosphohydrolase, was stimulated under these conditions (Vartanian et al.: Proceedings of the National Academy of Sciences of the United States of America 85:939, 1988) [ I 1. The possible role of cyclic AMP in activating specific phosphatases or restricting the availability of diacylglycerol for PK-C activation is discussed.

📜 SIMILAR VOLUMES

Differentiation of immature oligodendroc

Differentiation of immature oligodendrocytes is regulated by phosphorylation of cyclic AMP-response element binding protein by a protein kinase C signaling cascade

✍ Hatsuki Shiga; Yukako Yamane; Minoru Kubo; Yoko Sakurai; Hiroaki Asou; Etsuro It 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 283 KB

Previous experiments showed that the expression and phosphorylation levels of cyclic AMP-response element binding protein (CREB) are important factors that regulate oligodendrocyte differentiation. The present study was designed to determine whether CREB phosphorylation advances oligodendrocyte diff

Dual role of cyclic AMP-dependent protei

Dual role of cyclic AMP-dependent protein kinase in neuritogenesis and synaptogenesis during neuronal differentiation

✍ Takuro Tojima; Suguru Kobayashi; Etsuro Ito 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 323 KB

## Abstract To create precise neural circuits in the nervous system, neuritogenesis and synaptogenesis are the critical cellular processes during neuronal differentiation. We examined the cyclic AMP (cAMP)‐responsible signaling pathways for regulating neuritogenesis and synaptogenesis in NG108‐15 c

Regulation of the astrocyte resting memb

Regulation of the astrocyte resting membrane potential by cyclic AMP and protein kinase A

✍ Sally Bolton; Kirsty Greenwood; Nicola Hamilton; Arthur M. Butt 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 812 KB

## Abstract The factors regulating astroglial resting membrane potential (RMP) are unresolved. Here, we have examined developmental, morphological, and intracellular factors that may regulate the RMP in astrocytes of isolated intact optic nerves of rats and mice aged postnatal day (P3) to adult. Th

Activation of cyclic AMP-dependent prote

Activation of cyclic AMP-dependent protein kinase and stimulation of protein phosphorylation in response to adenosine in C-1300 murine neuroblastoma

✍ Green, Richard D. ;Noland, Thomas A. 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 590 KB

DEAE-cellulose chromatography of the 20,000g supernatant fraction of homogenates of C-1300 murine neuroblastoma (clone N2a) yields one major and two minor peaks of cyclic AMP-dependent protein kinase activity. Assessment of the endogenous activation state of the enzyme(s) reveals that the enzyme is

Cyclic AMP-dependent protein kinase A ne

Cyclic AMP-dependent protein kinase A negatively modulates adherens junction integrity and differentiation of intestinal epithelial cells

✍ Marie-Josée Boucher; Patrick Laprise; Nathalie Rivard 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 557 KB

## Abstract Intestinal epithelial cell differentiation is a complex process in which many different signaling pathways are likely involved. An increase in the intracellular levels of cyclic AMP (cAMP) has been shown to inhibit enterocyte differentiation; however, the mechanisms through which cAMP/P

Biphasic regulation of macrophage attach

Biphasic regulation of macrophage attachment by activators of cyclic adenosine monophosphate-dependent kinase and protein kinase C

✍ C. Issaad; M. A. Ventura; P. Thomopoulos 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 586 KB

A method is described that enabled us to study the adhesiveness of J-774 murine macrophages. Cell attachment was stimulated by activators of kinase C (i.e., phorbol esters) as well as kinase A (cyclic adenosine monophosphate; cAMP). This novel effect of cAMP was observed when its levels were increas