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Phosphoprotein electrophoresis in the presence of Fe(III) ions

✍ Scribed by Zdenka Kučerová; Hana Muselová; Petr Přikryl; Marie Tichá

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
193 KB
Volume
34
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Preparation of affinity polyacrylamide gels containing immobilized Fe(III) ions for the separation of proteins exhibiting metal ion binding properties is described. The presented method enables uniform distribution of immobilized metal ions in the affinity part of the polyacrylamide separating gel. Affinity gels prepared by this way are suitable to follow the effect of different concentrations of metal ions immobilized in polyacrylamide gel on a protein electrophoretic behavior. Polyacrylamide gels containing immobilized Fe(III) ions were used to study the electrophoretic behavior of two model proteins differing in their phosphate group content: chicken ovalbumin and bovine α‐casein. For the electrophoretic separation, both the native and the denaturating conditions were used.

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