Phosphoenolpyruvate carboxylase in avocado fruit: Purification and properties
β Scribed by Brian A. Notton; Michael M. Blanke
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 501 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0031-9422
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π SIMILAR VOLUMES
Phosphoenolpyruvate carboxylase (EC 4.1.1.31) was purified 43-fold from Amaranthus viridis leaves by using a combination of ammonium-sulphate fractionation, chromatography on O-(diethylaminoethyl)-cellulose and hydroxylapatite, and filtration through Sepharose 6B. The purified enzyme had a specific
## Abstract The activities of pyruvate carboxylase and phosphoenolpyruvate carboxykinase were measured in cultured human fibroblasts. Considerable variation was observed in strains derived from different individuals. The activities of both enzymes throughout the culture cycle were measured in two s