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Phosphocytidines as versatile 3′ protecting groups in triester synthesis of oligodeoxyribonucleotides

✍ Scribed by G.R. Gough; M.J. Brunden; P.T. Gilham

Book ID
104221267
Publisher
Elsevier Science
Year
1983
Tongue
French
Weight
261 KB
Volume
24
Category
Article
ISSN
0040-4039

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✦ Synopsis

Any oligodeoxyribonucleotide triester block with a 3' aryl phosphate end can be protected by condensing its phosphodiester N4,~3',~5'-tribensoylcytidine.

terminal with the 2'-hydroxyl group of As the final step in deprotection of the modified block, mild treatment with Pb* ion cleaves the new 3'-2' linkage in such a way that the original 3' terminal phosphate group of the oligomer is transferred to the ribonucleoside. This procedure eliminates the need for specialized terminator blocks in triester synthesis.

Biological applications of chemically synthesized oligodeoxyribonucleotides generally require that the molecules possess a free 3'-hydroxyl terminus. However, most synthetic

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