Phosphocellulose chromatography of human hemoglobins

✍ Scribed by Bennett F. Horton; Amoz I. Chernoff

• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 516 KB
• Volume: 63
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85663-6

✦ Synopsis

Ph&sphacelIuIase chromatqraphy of human hemoglobins

Phosphate compounds are known to bind to a number of proteins including hemoglobins. H~XSMAX and co-workers demonstrated a differential binding of inorganic phosphates to two adult chicken hemoglobins 1. More recently, it has been found that Hb A, Hb S, and Hb F have different binding affinities for 2.3~diphosphoglyceric acid2-4.

Since differences in affinities of hemoglobins for phosphate compounds esist, it seemed highly probable that phosphocellulose could be used for resolving various components of human hemoglobins. This paper, therefore, presents results of studies in which phosphocellulose was used both as an ion-exchange resin and as a stationary phosphate phase for column chromatography of human hemoglobins. Phosphocellulose chromatography separates the major hemoglobins into three subcomponents and separates Hb A, from Hb C.