Microchromatography of hemoglobins : V. Thin-layer chromatography of some hemoglobins on CM-cellulose

✍ Scribed by W.A. Schroeder; Norman C. Nelson

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 563 KB
• Volume: 115
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98956-3

✦ Synopsis

By means of thin-layer ion-exchange chromate_mphy, human hemoglobins A, S, and C in various combinations can be distinctly separated. Also, hemoglobin F can be separated from the A, S, and C components of cord bIood samples from infants and in a&_& blood samples with a rdativety high percentage of F (15 oA or more). The procedure uses CM-celEuIose and solutions of Tris-llCi, NaCI, and KCN A O-3-mg sample in 5-10 ,uI of solvent is required_ Development can be compfeted in 3Q43 min.

lNTRODt3CTiON

Akhough the thin-layer chromatography (TLC) of hemoglobins on such supports as SephadexzJ and Bio-GeP has been reported, these procedures would not separate norma! from abnormal hemoglobins because mokcular weights are virtually identical. There seem to be no descriptions of theTLC of hemoglobins on ion-exchange substances despite the fact that many procedures are known for the ion-exchange separation of hemoglobins in columns 44_ h%crochromatographic column methods have been describedX* +-I I_ Th e experiments to be reported here were designed to determine whether ion-exchange TLC of hemogIobins ofYered advantzges in speed, reduced quantity of material required, etc., in the qualitative detection of common abnotmai hemoglobins.

Samples were obtained from the Los Angeles Sickle Cell Center and the Los An&es County Health Department. They included both cord blood and adult speci-. kens wit& various combinations of hemoglobins A, S, C, and F.

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